In Vitro and In Vivo Effect of the Composition of Fucoxanthin with Porous Aluminum-Silicon Career on Cells

Abstract

Fucoxanthin natural carotenoid from seaweeds exhibits antioxidant properties. The paper analyzes the possibility of using fucoxanthin, immobilized on aluminum-silicon carrier particles, to assess the toxic effect on naïve or matured immune cells, enterocytes, Mesenchymal stem cells, and EA.Hy926 cell line. The proliferation, nitric oxide production, myeloperoxidase activity, and apoptosis of thymocytes and splenocytes of mice in vitro were studied. Proliferative capacity, nitric oxide production, and apoptosis rate of entreocytes, mesenchymal stem cells and EA.Hy926 cell line were investigated. It is shown that fucoxanthin immobilized on aluminum-silicon carrier particles increased the survival rate and proliferation of mature immunocytes (splenocytes) after 24 hours exposure and increased the survival rate of naïve immunocytes (thymocytes) when exposed 120 hours. On myeloperoxidase, the activity of immune cells is not affected by fucoxanthin immobilized on the carrier particles. Orally the administration of some samples of fucoxanthin immobilized on the carrier particles with different sizes, and dosage no have an adverse effect on jejunum enterocytes (proliferation and apoptosis). In vitro, all tested samples of fucoxanthin immobilized on the carrier particles with different sizes and dosage in common no have a toxic effect on jejunum enterocytes, human bone marrow mesenchymal stem cells, and EA.Hy926 cell line. The obtained results indicate that fucoxanthin, immobilized on particles of an aluminum-silicon carrier, does not have a toxic effect on mouse immunocytes and enterocytes, and human mesenchymal stem cells and EA.Hy92 cell line.