In vitro and in vivo coupling of Thiocapsa hydrogenase with cyanobacterial and algal electron mediators

Abstract

H 2 activation by the oxygen-tolerant hydrogenase from the purple sulfur bacterium Thiocapsa roseopersicina, using electron mediators of cyanobacterial and algal origin, has been demonstrated. Ferredoxins, either from the cyanobacterium Synechococcus PCC7942 or the green alga Scenedesmus obliquus, are capable of providing electrons for hydrogenase-mediated H 2 evolution. The high-potential cytochrome c 6 from Synechococcus PCC7942 proved to be capable of accepting electrons derived from hydrogenase-mediated H 2 oxidation. In subsequent experiments, Thiocapsa hydrogenase was introduced into the cells of Synechococcus PCC7942 by electroporation, for enhanced H 2 production.