Abstract
Enterotoxigenic Escherichia coli (ETEC) and Shigella ssp. infections are associated with high rates of mortality, especially in infants in developing countries. Due to increasing levels of global antibiotic resistance exhibited by many pathogenic organisms, alternative strategies to combat such infections are urgently required. In this study, we evaluated the stability of five coliphages (four Myoviridae and one Siphoviridae phage) over a range of pH conditions and in simulated gastric conditions. The Myoviridae phages were stable across the range of pH 2 to 7, while the Siphoviridae phage, JK16, exhibited higher sensitivity to low pH. A composite mixture of these five phages was tested in vivo in a Galleria mellonella model. The obtained data clearly shows potential in treating E. coli infections prophylactically.
Highlights
Diarrheal diseases are among the leading causes of mortality in children under the age of five years [1,2]
diarrheagenic Escherichia coli (DEC) are currently classified into six groups: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), and diffusely adhering E. coli (DAEC) [4]
We evaluated the ability of five distinct phages to survive simulated gastric conditions
Summary
Diarrheal diseases are among the leading causes of mortality in children under the age of five years [1,2] Most of these deaths occur in so-called developing countries, especially sub-Saharan Africa and southern Asia [1,3]. DEC are currently classified into six groups: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), and diffusely adhering E. coli (DAEC) [4]. These pathotypes differ from each other in terms of the clinical and pathological characteristics of the infection and in the prevalence in various countries and regions [5,6,7]. According to the European Centre for Disease Prevention and Control (ECDC), in 2018, an excess of 8000 cases of EHEC infections were reported in the EU
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