In vitro and in vivo antiproliferative activity of arucanolide isolated from Calea pinnatifida

Abstract

Arucanolide is a germacranolide, isolated from the dichloromethane leaves' crude extract. Germacranolides are frequently described as responsible for antiproliferative activities with apoptosis induction. Therefore, study of anticancer activity of C. pinnatifida extracts and substances became important. This compound was identified as involved with the antiproliferative activity by bio-guided assays evaluated in nine different tumor and one normal cell lines. Samples were tested in a concentration range from 0.25 to 250μg/ml and activity was measured by sulforrodamine B method and total growth inhibition was calculated by non linear regression. The most potent fraction showed high selectivity for melanoma (TGI5: 1,04μg/mL; TGI6: 1,07μg/mL) and kidney (TGI5: 1,45μg/mL; TGI6: 1,32μg/mL) cancer cell lines. The dichloromethane leaves' crude extract demonstrated In vivo activity when evaluated in solid and ascitic Erhlich tumor assay. Purification of this fraction by column chromatography afforded Arucanolide that was identified by comparison of the spectral data with literature [1].