In vitro and in vivo anticancer studies of n-butanol fraction from ethanol extract of annona muricata leaves

Abstract

Background: Cancer is a leading life threatening disease that adversely affects millions of people in a year worldwide. The growing concerns on the impact of cancer treatment such as surgery, radio & chemotherapy has been notably redirected to the usage of natural bioactive compounds having potential anticancer activity. Annona muricataLinn, a tropical plant belonging to Annonaceae family, was broadly investigated for the ethno medical property. The leaves of the plant was found to contain acetogenins that showed the significant anticancer activity. Objectives: To employ n-butanol fractionation to get the abundance of alkaloid phytochemicals from ethanolic extract of Annona muricata leaves and to study the extract In- vitro and In-vivo for anticancer activity. Methods: MTT assay was performed in-vitro to analyze the cytotoxicity of the extract.The invivo studies were performed with albino mice and the effect of n-butanol extract was studied on the body weight, hematological & biochemical parameter, hepatic enzymes, antioxidant proteins of the experimental animals. Results: The cytotoxic potential of the n-butanol extract using MTT assayshowed the IC50 value of 50 µg/mL inferring 50% of inhibition to MCF-7 adenocarcinoma cells.