In vitro and in vivo anthelmintic effects of Sterospermum kunthianum (Cham-Holl) leaf extract against Ascaridia galli in experimentally infected broiler chickens.

Abstract

This study was to assess the anthelminthic potential of Sterospermum kunthianum leaf extract against Ascaridia galli in experimentally infected broiler chickens. The extract and fractions were evaluated for in vitro inhibition and in vivo anthelmintic effects. Acute toxicity studies of extract revealed no sign of toxicity or death in birds at oral dose range of 1000-5000 and was considered safe. There was a concentration dependent decrease on inhibition of A. galli egg embryonation and deparasitization. At 100mg/ml, albendazole (ALB) caused the highest inhibition of embryonation (195.3 ± 0.9) which was not significantly different from the decrease caused by crude methanol extract (CME) (188 ± 0.9), hexane fraction (HF) (177 ± 1.2) or ethyl acetate fraction (EAF) (168.3 ± 0.9). The highest inhibition rates (%) were 97, 94, 88 and 85 for ALB, CME, HF and EAF, respectively. The deparasitization obtained at day 21 in ALB (95.62%) treated birds was not significantly (P > 0.05) different from the 81.27% and 89.24% obtained from the crude methanol treated birds. The deparasitization caused by CME at 400mg/kg (89.24%) was significantly higher than the one caused by EAF at the same dose (50.19%). Day 21 post treatment, significantly (P < 0.05) higher deparasitization was recorded for CME and HF at dosage of 400mg/kg when compared to 200mg/kg. Histopathology findings revealed necrosis of the mucosal gland and villi in chickens. In conclusion, the leaf extract and fractions S. kunthianum have been shown to possess anthelmintic activity.