Abstract
An immunostimulatory factor was identified 5o be secreted by antigen-pulsed macrophages. This factor was able to induce the generation of antigen specific T helper lymphocytes in vitro as well as in vivo. Further in vitro experiments testing for the genetic restriction of this factor indicated that it is a genetically-restricted antigen specific factor (ASF). The Cunningham plaque assay was used to quantify the generation of T helper lymphocytes by measuring the number of plaque forming cells after sequential incubations of antigen-pulsed macrophages with T lymphocytes, and then spleen cells, and finally the TNP-coated sheep red blood cells.
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