In vitro and in silico study to evaluate the effectiveness of quercitrin as antiviral drug to dengue virus

Abstract

Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.