In Vitro and In Silico Interaction Studies with Red Wine Polyphenols against Different Proteins from Human Serum.

Raja Mohamed Beema Shafreen,Shela Gorinstein,Selvaraj Alagu Lakshmi,Joseph Deutsch,Elena Katrich,Young-Mo Kim,Shunmugiah Karutha Pandian

doi:10.3390/molecules26216686

Raja Mohamed Beema Shafreen, Shela Gorinstein + Show 5 more

Open Access

https://doi.org/10.3390/molecules26216686

Copy DOI

Abstract

Previous reports have shown that consumption of wine has several health benefits; however, there are different types of wine. In the present study, red wines were investigated for their compositions of active ingredients. The interaction of each component in terms of its binding mode with different serum proteins was unraveled, and the components were implicated as drug candidates in clinical settings. Overall, the study indicates that red wines have a composition of flavonoids, non-flavonoids, and phenolic acids that can interact with the key regions of proteins to enhance their biological activity. Among them, rutin, resveratrol, and tannic acid have shown good binding affinity and possess beneficial properties that can enhance their role in clinical applications.

Highlights

Phenolic compounds are an essential part of the human diet and are of considerable interest due to their antioxidant properties and potential beneficial health effects
Previous reports have shown that the consumption of wine has several health benefits
There are different types of wine; in the present study, red wine was investigated for its composition of active ingredients

Summary

Introduction

Phenolic compounds are an essential part of the human diet and are of considerable interest due to their antioxidant properties and potential beneficial health effects. Research on wine has been widely cited, but in spite of a number of reports [17,18] and evidence that wine exerts beneficial effects on human health when it is consumed with moderation, how the main phenolics of red wine contribute to the quenching properties of the main human serum proteins has not been investigated For this reason, red wine samples were investigated for their antioxidant activities, bioactive compounds, and interactions of wine polyphenols with the main serum proteins, with an emphasis on the influence of polyphenols and not the alcohol content on the health properties of wine. Fluorescence measurements, antioxidant assays, and docking analysis were applied for the first time to wine samples, and correlations were made among the results obtained in these studies

Methods

Discussion

Conclusion