In-Vitro and in-Silico characterization of Sophora interrupta plant extract as an anticancer activity.

Pardhasaradhi Mathi,Kumar Nikhil,Venkata Raman Bokka,Mahendran Botlagunta,Partha Roy,Nagavamsikrishna Ambatipudi

doi:10.6026/97320630010144

Abstract

Sophora interrupta belongs to the family of Fabaceae and the species in this genus have a diverse medicinal importance as a folk medicine for preventing many ailments including cancer. In order to evaluate the anticancer activity of S.interrupta, we have performed in vitro anti-oxidant, anti-inflammatory, anti-proliferative, and cell based anticancer activity in MCF-7 and PC-3 cell lines. Secondary metabolites of S.interrupta were used to identify anticancer compounds using Open Eye software. The antioxidant activity of the S.interrupta root ethylacetate (SEA) extract at 100 µg/ml is equal to that of ascorbic acid at 50 µg/ml. The antiinflammatory activity of SEA is half of that of diclofenac at 50 µg/ml. Anticancer activity was detected by measuring the mitochondrial dehydrogenase activity (MTT assay). The half maximal inhibitory concentrations (IC50) for MCF-7 and PC-3 cell lines are 250 and 700 µg/ml respectively. This was supported by the morphological changes such as membrane blebbing, cell detachment and rounded cell morphology when compared to the parental cells. In addition, we observed few green cells (live) over red cells (dead) based on the uptake of acridine orange and ethidium bromide dyes. Kaempferol-3-O-b-D-glucopyranoside, a Secondary metabolite of S.interrupta form 6 hydrogen bond interactions with Arg 202, Gln 207, Gly 227, Gly 229, Thr 231 and Ala 232 human DEAD box RNA helicase, DDX3 protein and is equivalent to crystal structure of adenosine mono phosphate to DDX3. Overall, it suggests that the SEA extract has anticancer compounds, and it can be used to enhance death receptor mediated cancer cell death.

Highlights

Cancer is the major leading cause of deaths in all over the globe and the etiology of cancer is linked to several lifestyle choices in the modern society
3ml of Phosphomolybdate reagent is mixed with a series of 300 μl of S.interrupta root ethylacetate (SEA) of the plant in a test tube
The tubes were cooled down to room temperature, and the absorbance measured at 695 nm against the blank

Summary

Introduction

Cancer is the major leading cause of deaths in all over the globe and the etiology of cancer is linked to several lifestyle choices in the modern society. These lifestyle choices include cigarette smoking, excessive alcohol consumption, poor diet, lack of exercise and hormones [1]. Chemotherapy is most commonly used method to treat metastatic cancer using cytotoxic drugs [3] Those agents often have shown to generate side effects by affecting the cell viability of both tumor and normal cells, thereby bring down the quality of human life [4]. Several anticancer compounds have been derived from natural resources including

Methods

Results

Conclusion