In vitro analysis of green fabricated silver nanoparticles (AgNPs) against Pseudomonas aeruginosa PA14 biofilm formation, their application on urinary catheter

Abstract

Methanolic (SME) extract of the biologically safe and edible cyanobacteria, Spirulina platensis was used for the green fabrication of silver nanoparticles (AgNPs). Characterization using UV–vis spectra showed the absorbance at 420 nm confirming the successful synthesis of AgNPs. Further characterization using XRD, DLS, SEM and TEM analysis showed the synthesis of stable (-43.6 mV), spherical and dispersed Ag nanoparticles with an average size of 29 nm. Antibiofilm potential of AgNPs (10, 25, 50, 75 and 100 μg mL−1) was tested against Pseudomonas aeruginosa PA14 showed a maximum of 85.63 % inhibition of biofilm formation at 100 μg mL−1. Phenotypical change in the biofilm morphology was confirmed using light and confocal microscopic analysis. Inhibiting the production of rhamnolipid by P. aeruginosa was observed at the concentration of 25 μg mL−1. Further application of AgNPs to prevent biofilm formation in urinary catheters were tested against P. aeruginosa PA14. Thus, the present study will promote an idea to use AgNPs synthesized using SME for coating medical device and prevent biofilm associated infections and diseases.