Abstract
Most plants are considered to prevent free radicals associated damages by numerous ways including direct scavenging of free radicals and inhibition of enzymes involved in free radical production. The aim of the present study was to examine the antioxidant activities of the hexane from polyherbal plants of Carica papaya, Punica granatum, Catharanthus roseus and Cympbopogan citratus which is long been known to be a very important source of pharmaceutical. Different concentrations of polyherbal extract was tested for anti-oxidant properties using hydrogen peroxide assay. Similarly, the free radical scavenging activity was determined by DPPH assay. The results showed that the polyherbal extract showed a strong inhibition value at IC50 of concentration of 170.7 μg/ml, 229.57 μg/ml. This study provides evidence that the polyherbal formulation possesses a strong antioxidant activity. Therefore, it might be beneficial as medicinal plant as an antioxidant and anticancer nutraceutical and pharmaceutical sources. Keywords: Antioxidant activity, DPPH assay, Hexane, Hydrogen peroxide assay
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