Abstract
Irrigant solutions commonly used for the treatment of endodontic infections can be inhibited by both organic and inorganic substances. The aim of this study was to evaluate the in vitro antimicrobial activity of the novel irrigant HybenX® and 2.5% and 5% sodium hypochlorite against Enterococcus faecalis, in presence of dentine powder (DP) or bovine serum albumin 20% (BSA) as inhibitory agents. An E. faecalis American Type Culture Collection (ATCC) 29212 suspension was added to the irrigants (Hybenx® or NaOCl) and one or two different inhibitors (BSA and DP) either after one-hour pre-incubation at 35 ± 1 °C or not. The antimicrobial activity of HybenX® against E. faecalis was already proved at 15 min and was neither affected by BSA nor by DP or combinations thereof. NaOCl 2.5% showed an effective antimicrobial activity starting from 15 min and this activity was partially inhibited by BSA and BSA plus DP combination within one hour when pre-incubation occurred. NaOCl 5% showed antimicrobial activity within 15 min, which was inhibited within one hour only in the presence of both BSA and DP regardless of the pre-incubation period. HybenX® could represent a good alternative to common irrigants for the treatment of E. faecalis endodontic infections, showing a rapid antimicrobial activity not inhibited by organic and inorganic inhibitors.
Highlights
The success of endodontic treatment is strongly influenced by the control of residual intracanal infections and the prevention of reinfections [1,2,3]
The presence of dentine powder (DP) and/or bovine serum albumin (BSA) did not affect E. faecalis viability, and survival curves in their presence were comparable with that observed in normal saline
The presence of BSA or DP had no inhibitory effect on the bactericidal activity of HybenX®, even after a pre-incubation step with the potential inhibitors (Figure 1)
Summary
The success of endodontic treatment is strongly influenced by the control of residual intracanal infections and the prevention of reinfections [1,2,3]. For this reason, irrigant solutions with bactericidal activity are used during endodontic treatment. Irrigant solutions used in the endodontic treatment may exhibit an excellent bactericidal activity when used in vitro, with rapid bacterial killing at low concentrations. Many factors, such as the presence of organic compounds (e.g., albumin) present in inflammatory exudate and hydroxylapatite (main inorganic component of dentine) can reduce the antibacterial efficacy of irrigant solutions [6]. The co-administration of different irrigant solutions can show limited benefits [7]
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