In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats

Mohamed Akram Melakhessou,Salah Eddine Marref,Latifa Khattabi,Cherine Marref,Imene Becheker,Naima Benkiki

doi:10.1186/s43094-021-00358-5

Mohamed Akram Melakhessou, Salah Eddine Marref + Show 4 more

Open Access

https://doi.org/10.1186/s43094-021-00358-5

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Abstract

BackgroundDiabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug.ResultsThe BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively.ConclusionBEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.

Highlights

Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies
The α-Glucosidase inhibitory effect of the extract was more efficient than α-Amylase inhibition with 89.37% at 1000 μg/mL
In vivo antidiabetic activity Oral Glucose Tolerance Test (OGTT) in rats As indicated in Fig. 2, there were no significant differences in blood glucose level among all the groups (P > 0.05) before glucose administration

Summary

Introduction

Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. This work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. Diabetes is a chronic disease associated with elevated blood glucose levels, triggered by the combination of hereditary and environmental sources which cause an abnormally high blood sugar level [1] It is a major health issue in both developed and undeveloped countries [2]. The presence of narcissin, tiliroside, ladaneine, and vicenin was confirmed in the n-butanol extract [11] Among these phytochemical constituents, tiliroside and vicenin have been shown to exhibit antidiabetic activity [12, 13]. This study was to investigate whether the n-butanol extract of the whole plant A. flava possessed antidiabetic activity or not

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