Abstract

In vitro exposure of homogeneous monolayer cultures of thioglycollate-elicited mouse peritoneal macrophages to glucocorticoid hormones for up to 96 hr produced a progressive, dose-related inhibition of cell growth and protein synthesis. The reductions in cell protein, incorporation of radiolabeled leucine, and intracellular water space were specific to steroids with glucocorticoid action, with half-maximal inhibition occurring at about 10(-9) M for dexamethasone. Similar dose-related, glucocorticoid-specific decreases in net glucose uptake, lactate production, and 14CO2 production were observed that were only partially explained by the decrease in cell mass. A decrease in the monosaccharide transport system Vmax was also noted but could be completely accounted for by the reduction in cell surface area and the resulting proportional loss in transport sites. No effect on transport system affinity could be demonstrated. The suggestion was made that such long-term culture of macrophages in the presence of glucocorticoids reverses the biochemical and functional changes that comprise the "activated" state of mononuclear phagocytes.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.