Abstract
This study was designed to investigate the effect of Artemisia capillaris extracts on the intestinal microflora. In agar diffusion method, the solvent fractions of Artemisia capillaris showed growth inhibition against the intestinal microflora. In particular, the chloroform fraction of Artemisia capillaris had strong antibacterial activity against Clostridium perfringens, Clostridium difficile, Eubacterium limosum, and Bacteroides fragilis, but did not show antibacterial activity against Bifidobacterium bifidum and Lactobacillus acidophilus. Most chloroform fraction of Artemisia capillaris inhibitory activities were not reduced by heat treatment or pH variation against C. perfringens, C. difficile, E. limosum, and B. fragilis. MICs of the chloroform fraction were 1.25 ㎎/mL against C. perfringens, E. limosum and B. fragilis and 2.5 ㎎/mL against C. difficile. MBCs of chloroform fraction were 5 ㎎/mL against C. perfringens, E. limosum and 2.5 ㎎/mL against C. difficile, B. fragilis. The ethyl acetate fraction of Artemisia capillaris showed 3.08±0.03 ㎎/10 ㎎ total polyphenol and 1.91±0.03 ㎎/10 ㎎ total flavonoid contents. In vivo tests were performed to investigate the influence of Artemisia capillaris extract on the intestinal microflora in rats. The results showed the possibilities of utilizing Artemisia capillaris extracts as a functional food component to control intestinal microflora.
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