Abstract
Many studies of proximal tubule function require information about the elemental composition in subcellular compartments that can be obtained by electron probe x-ray microanalysis (EPXMA). While experiments of this type have been performed on isolated proximal tubule preparations, it is also necessary to conduct parallel experiments on intact tissue to verify those results obtained with isolated tubules. One limitation in conducting EPXMA analysis on whole tissue is obtaining adequately cryopreserved tissue. Metal mirror cryofixation techniques have yielded excellent preservation in dissected tissues or cell cultures. Therefore, experiments were performed to assess the efficacy ofin situmetal minor fixation of the intact rat kidney.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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