Abstract
The development of cryo-focused ion beam (cryo-FIB) microscopy into a highly reliable sample preparation technique for frozen-hydrated specimens has recently enabled cryo-electron tomography (CET) of eukaryotic cells with unprecedented resolution and image quality [1,2,3]. The ability to prepare distortion-free lamellas of homogenous, user-defined thickness has allowed in situ studies of cellular structures in their native state, revealing new insights into biological processes [4]. Additionally, cryo-fluorescence microscopy has been combined with cryo-FIB for the in situ targeting of fluorescently-labelled cellular structures [5].
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