Abstract
There is an increasing interest in real-time and in situ monitoring of living cell activities in life science and medicine. This paper reports a whole cell sensing protocol over the interface of Au film coupled in a wavelength-modulated surface plasmon resonance (WMSPR) biosensor. With dual parabolic mirrors integrated in the sensor, the compact and miniaturized instrument shows satisfactory refractive index sensitivity (2220 nm/RIU) and a high resolution of resonance wavelength shift of 0.3 nm to liquid samples. The affinity interactions between the biomarker of human tumor endothelial marker 8 (TEM8) and antibody (Ab) or specific polypeptide (PEP) were firstly introduced to WMSPR biosensor analysis. Both the interaction events of Ab-cell and PEP-cell over the Au film interface can be recognized by the sensor and the balance time of interactions is about 20 min. The concentration range of Ab for quantitative monitoring of the TEM8 expression on human colon carcinoma SW620 cells was investigated. The present low-cost and time-saving method provides a time resolution of binding specificity between Ab/PEP and TEM8 for real-time analysis of antigen on living tumor cell surface.
Highlights
There is an increasing interest in real-time and in situ monitoring of living cell activities in life science and medicine
The main purpose of this paper is to demonstrate the feasibility to target living cell surface receptor of tumor endothelial marker 8 (TEM8) using wavelength-modulated surface plasmon resonance (SPR) biosensor
The affinity interactions between the newly discovered biomarker of TEM8 and specific polypeptide were firstly introduced to SPR analysis
Summary
There is an increasing interest in real-time and in situ monitoring of living cell activities in life science and medicine. The affinity interactions between the biomarker of human tumor endothelial marker 8 (TEM8) and antibody (Ab) or specific polypeptide (PEP) were firstly introduced to WMSPR biosensor analysis Both the interaction events of Ab-cell and PEP-cell over the Au film interface can be recognized by the sensor and the balance time of interactions is about 20 min. Since Giebel K.F. applied this technique in living cell analysis for the first time[22], SPR has been widely used to characterize various cellular processes[23], including cell morphology changes[24], cellular response to environmental stress[25], cell-protein interactions[26,27,28] and other cellular activities[29] This is mainly due to its advantages in label-free and real-time analysis of living cells, which are significantly important for cell based drug screening and evaluation
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