In-situ synthesis of copper-gallic acid metal–organic framework into the gentamicin-loaded chitosan hydrogel bead: A synergistic enhancement of antibacterial properties

Abstract

In the history of modern medicine, there has been a serious risk of antibacterial drugs as losing their effectiveness against bacteria. In this regard, nanocomposite hydrogel beads based on biopolymers such as chitosan (CS) have been recently developed as antibacterial platform. In this work, antibacterial copper-gallic acid metal–organic framework (GA-MOF) were developed to increase the biological efficiency of CS hydrogel beads. To end this, in-situ synthesis method was used to grow different concentration of GA-MOF into the hydrogel matrix. Also, gentamicin (GM) as the antibacterial drug was preloaded in the preparation of CS hydrogel beads to further enhance the antibacterial properties. Various analysis techniques were utilized for the validation of successful nanocomposites construction. In-vitro GA and GM release in the simulated physiological environmental (PBS, pH 7.4) were considered, revealing a controlled and sustained release profile during 72 h. Antibacterial studies showed inhibition zone of 26 ± 0.5 and 18 ± 0.5 mm for the Staphylococcus aureus and Escherichia coli bacteria, respectively. In addition, the MTT assay demonstrated good cytotoxicity (over 70 % cell viability after 48 h) for the human skin fibroblast HFF-2 cells. According to the results, the prepared nanocomposites have a good potential for use as an antibacterial bioplatform.