Abstract
A better understanding of the molecular mechanisms that control the UCP1 expression in brown and beige adipocytes is essential for us to modulate adipose cell fate and promote thermogenesis, which may provide a therapeutic view for the treatment of obesity and obesity-related diseases. To systematically identify cis-element(s) that transcriptionally regulates Ucp1, we here took advantage of the high-throughput CRIPSR-Cas9 screening system, and performed anin situsaturating mutagenesis screen, by using a customized sgRNA library targeting the ~ 20kb genomic region near Ucp1. Through the screening, we have identified several genomic loci that may contain key regulatory element for Ucp1 expression in cultured brown and white adipocytesin vitro, and in inguinal white adipose tissuein vivo. Our study highlights a broadly useful approach for studying cis-regulatory elements in a high-throughput manner.
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