In situ purification of ammonium nitrogen wastewater in rare earth mine by native bacteria isolating from original mining area

Abstract

Ammonium sulfate ((NH4)2SO4) leaching method to extract rare earth elements (REEs) of mine has produced a large amount of NH4+-N-enriched wastewater derived from ore body, leading to many serious environmental pollution problems. This study was the first time to establish an in-situ treatment for real REEs wastewater outside and inside the ore body by an isolated indigenous microorganism. The results stated that Citrobacter sp. X-9 achieved the highest NH4+-N removal efficiency among the isolated six microbial strains. Moreover, the microbe to treat the REEs wastewater outside ore body gave the greatest NH4+-N removal efficiency under the optimized conditions in the Erlenmeyer flask (250-mL) and bioreactor (10-L). Furthermore, compared to the others’ modes, the in-situ treatment by cyclic mode with Citrobacter sp. X-9 possessed superior performance in NH4+-N removal efficiency for wastewater inside of ore body, showing that the established in-situ treatment was the potential approach for REEs wastewater purification.