Abstract
It is believed that in situ nick end-labeling (ISNEL) is an easy and selective method for detecting apoptosis in situ. To test whether ISNEL selectively detects apoptosis but not necrosis, we investigated the kainic acid (KA)-induced neuronal death with ISNEL, comparing with the results of gel electrophoresis and electron microscopy. Many degenerating neurons (ca. 50%) in the hippocampal CAI area and amygdaloid complex were intensely stained with ISNEL 1–3 days after intraperitoneal injection of KA. Although most of the ISNEL-positive neurons displayed a pathological feature of necrosis, a small number of them displayed apoptosis-like changes when examined by electron microscopic observation. It should be noteworthy that ISNEL recognizes at least a certain form of necrosis and is not selective for apoptosis.
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