Abstract
AbstractPhotothermal biosensors have emerged as an attractive strategy for point‐of‐care testing (POCT). However, developing a photothermal biosensor with good accuracy to realize high‐throughput detection is still a big challenge. Herein, a photothermal biosensor comprised of a paper‐supported wettable microchip was proposed for sensitive, accuracy, high‐throughput detection of microRNA (miRNA). Hybridization chain reaction (HCR) products on the wettable paper induced by target miRNA were used as a template for in situ formation of Ag2S nanoparticles (NPs), which can translate miRNA concentration into temperature change. The developed photothermal biosensor with low sample volume exhibited high sensitivity with a detection limit of 49.7 pM. In addition, the biosensor showed excellent reproducibility and selectivity, and the miRNA content in cell lysates was successfully assayed. The proposed photothermal biosensor shows great potential in biomedical application and clinical diagnosis.
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