Abstract
Enzymes with high catalytic activity and stability have been used for the sustainable development of green chemical applications, such as water remediation. Immobilized laccase can be used to construct a synergistic system for adsorption and degradation, which has great potential for water remediation. Herein, a hydrogen-bonded organic framework was installed onto laccase in-situ to form a net-carboxylate-arranged defective cage, which enhanced its catalytic stability. Thereafter, the CMC/PVA/Lac@HOF-101 hydrogel was fabricated by freeze–thaw cycles using sodium carboxymethylcellulose and polyvinyl alcohol as carriers and copper (II) as a cross-linker. Notably, the MOFs/hydrogel as a protective carrier of laccase maintain long-term recyclability and catalytic stability. After the fifth catalytic cycle, approximately 66.7 % activity of the CP-Lac@HOF-101 was retained. When both free laccase and CP-Lac@HOF-101 were used for decolorization of Acid Orange 7 (AO), the removal rates were 10.9 % and 82.5 % after 5 h, respectively. Furthermore, even in the presence of metal cations, almost 60.0 % of the AO removal efficiency was achieved. The relationship between the structure of the azo dyes and decolorization efficiency of the synergistic system was further investigated. This study offers a method for constructing enzyme@HOF-based composite hydrogels and provides a promising water remediation strategy.
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