In situ elimination of nitrite inhibition on AnAOB by acetate dosing in an up-flow granular anammox reactor

Abstract

The inhibition of over-accumulated nitrite on anaerobic ammonium oxidation (anammox) activity has been widely reported and intensively studied. Surprisingly, there are limited researches on the strategy to deal with nitrite inhibition. In this work, to eliminate nitrite inhibition in an up-flow granular anammox reactor, acetate dosing (600 mg COD L−1) and simultaneous acetate and denitrifying sludge dosing (600 mg COD L−1 and 1.4 g dry weight L−1) were implemented to temporarily activate microbial denitrification to reduce nitrite, respectively. In two strategies, reactor nitrogen removal and extracellular ATP were resumed to initial levels, while the recovery ratios of intracellular ATP and nitrite removal rate (67.1% and 15.6 mg N h−1) of the former were higher than those (52.5% and 11.2 mg N h−1) of the latter, indicating acetate dosing was more qualified to nitrite removal. Meanwhile, although a decrease of the dominated Ca. Brocadia from 30.7 to 25.8% was not reversed through high-throughput sequencing, acetate dosing did not cause denitrifiers proliferation. As easily implemented acetate dosing was as effective as direct discharge of inhibitory nitrite as the control strategy, it was recommended when nitrite inhibition happened. Additionally, an irregular behavior of nitrate overproduction via nitrite oxidation and the drastic increase of extracellular ATP were detected and proposed as the response of AnAOB to nitrite inhibition.