In Situ Dynamics of Aromatic Hydrocarbons and Bacteria Capable of AH Metabolism in a Coal Tar Waste-Contaminated Field Site

Abstract

Field experiments utilizing randomized block designs were implemented to assess the mobility of both coal tar-derived aromatic hydrocarbons and bacteria capable of metabolizing these substances at a contaminated field site. Arrays of sorbent materials wrapped in fiberglass mesh fabric were inserted into organic matter-rich freshwater sediments in order to intercept mobile chemicals and bacteria carried by the prevailing hydraulic gradient. Polyurethane foam plugs served as a sorbent for aqueous-phase coal tar components while sterile sand from the site served as a substrate for colonization by bacteria. Replicate sorbents were removed from the sediments at varying intervals and assessed for organic compounds (via gas chromatography/mass spectrometry) and for numbers of aromatic hydrocarbon-degrading bacteria (via viable plate counts). Organic contaminants including naphthalene, methyl naphthalene, indenes, and substituted benzenes were detected in the foam sorbents. Contaminant concentrations reached a maximum after 15 days before diminishing. Both naphthalene- and phenanthrene-utilizing bacteria were mobile and reached peak titers of 104 and 103.3, respectively, within 11 days. Thus, comigration of both contaminants and microorganisms occurred at the study site. Furthermore, the in situ abundances of contaminants and microorganisms reflect a dynamic balance between processes causing accrual and elimination.