Abstract
In situ hybridization has provided insights into the molecular basis of skeletal myogenesis during embryonic development. In situ detection of different muscle-specific regulatory factors in whole embryos has been described. Spatial and temporal expression patterns of these factors differed among species. The expression pattern of MyoD in whole chicken embryos was studied via in situ hybridization using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique. In newly formed somites (embryos of stage 12), MyoD mRNA transcripts were detected along the anterior to posterior axis of somites immediately adjacent to the neural tube, whereas in mature somites (embryos of stage 24), MyoD transcripts were detected throughout the entire somite. These results indicate that MyoD expression is important for initiating and maintaining the avian myogenic system.
Highlights
Skeletal myogenesis during embryonic development involves a large spectrum of gene activation and cellular signalling mechanisms
Recent studies of the molecular mechanisms involved in skeletal muscle development have shown that myogenesis is strictly dependent on a family of muscle-specific transcription factors, including MyoD, myogenin, myf-5 and MRF-4 (Emerson Jr., 1993; Cossu et al, 1996)
We investigated the transcript expression pattern of the myogenic factor MyoD in whole chicken embryos in in situ hybridization analysis using a probe obtained by the reverse transcription - polymerase chain reaction (RT-PCR) technique
Summary
Skeletal myogenesis during embryonic development involves a large spectrum of gene activation and cellular signalling mechanisms. Recent studies of the molecular mechanisms involved in skeletal muscle development have shown that myogenesis is strictly dependent on a family of muscle-specific transcription factors, including MyoD, myogenin, myf-5 and MRF-4 (Emerson Jr., 1993; Cossu et al, 1996). In situ analyses of mouse embryos have shown that in somites myf-5 is expressed first, followed sequentially by myogenin, MRF-4, and MyoD (Bober et al, 1991; Hinterberger et al, 1991).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
