Abstract
Abstract Fluorescent probes are indispensable tools for detection of enzymatic activities. However, detection of enzymes with high substrate specificity with substrate-based fluorescent probes is still challenging, due to their relatively large molecular size. We report herein enzyme assays coupled with a bioorthogonal fluoride ion-induced desilylation of fluorogenic probe. This sensing system is useful for in situ detection of enzyme reactions with high substrate specificity.
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