In-situ degradation of Amphotericin B in a microbial electrochemical cell containing wastewater

Abstract

Antimicrobial resistance raises serious medical implications and is primarily caused by indiscriminate usage and environmental contamination with antimicrobial agents. To prevent microbes from developing resistance against antimicrobial agents, they must be effectively degraded. This is the first study that investigates the degradation of Amphotericin B(AmB) with simultaneous wastewater treatment in a Microbial Peroxide producing cell (MPPC). Two sets of MPPCs (A and B) were used to degrade AmB oxidatively, one with H2O2 and the other with the microbial electro Fenton process in a catholyte containing 0.1% AmB. MPPC A and B had voltage outputs of 0.356 ± 3 V and 0.411 ± 2 V, producing 26 ± 0.04 mM and 44 ± 0.8 mM of H2O2 respectively. The structural changes of treated samples were analyzed using Fourier Transformed Infrared Spectroscopy, which revealed the disappearance of major characteristic bands such as the NH band (1556 cm−1), the CH band Polyene ring (3358 cm−1), and others, implying the disruption of multiple double bonds in polyene, resulting in the structure's lactone ring breakdown. Liquid chromatography quadrupole time-of-flight revealed the changes in retention time and peak area of treated samples in comparison to native AmB which also confirmed its structural changes. Such structural disruption induced the drug to lose its antifungal action since no zones of inhibition were detected in an antimicrobial susceptibility test against Candida albicans. The degradation of 57.05% and 69.83% of AmB by H2O2 and the Fenton process was also correlated with a reduction in COD. Simultaneously the anodic wastewater treatment in both the MPPCs had COD removal efficiency of 78% and 82% and the BOD removal efficiency was 75.38% and 90% respectively. The MPPC system's process conditions and reactor design could be optimized further to enhance antimicrobial degradation and wastewater treatment. This research offers a sustainable and efficient method for expediting antimicrobial degradation while simultaneously treating wastewater.