In situ cell electrospun using a portable handheld electrospinning apparatus for the repair of wound healing in rats.

Abstract

Slow or non-healing wounds caused by full-thickness skin wounds of various origins have become a difficult challenge in clinical wound treatment. In particular, large full-thickness skin wounds often lead to serious chronic skin wounds that do not heal. Electrospinning technology and stem cell treatment for wound repair have attracted much attention due to its unique advantages. In the current study， we electrospun polyvinyl alcohol (PVA) and bone marrow-derived stem cells (BMSCs) by a handheld electrospinning device, the distribution and interaction of cells and fibres were determined by light and electron microscopy and the cell viability and proliferation were determined by live/dead cell staining. The tissues were analysed by histology with Haematoxylin and Eosin (H&E) and Masson staining and immunohistochemical staining. We found that the fibres were distributed uniformly and BMSCs were distributed between the fibres. Cytotoxicity and cell proliferation tests proved its good biocompatibility. Histological staining shows it can accelerate wound healing and appendages regeneration by promoting granulation tissue repair. The instant PVA/stem cell fibres prepared by a handheld electrospinning device strongly promote the repair of full-thickness skin wounds in rats. The proposed electrospinning technology is expected to have great potential in household, outdoor and battlefield first aid.