In situ cell depletion of some marine algae enclosed in dialysis sacks and their use for the determination of nutrient-limiting growth in Ligurian coastal waters (Mediterranean sea)

Abstract

During the late spring and early summer 1976, laboratory cultured cells of five marine algae were enclosed in dialysis sacks and grown in situ, in Ligurian coastal waters. Skeletonema costatum (Greville) Cleve did not grow at all. Hemiselmis virescens Droop stayed alive, but did not grow significantly. Platymonas suecica (Butcher) Manton & Parke grew on cell reserves. Only Phaeodactylum tricornutum Bohlin and Thalassiosira pseudonana Hasle & Heimdal took up nutrients from sea water when all reserves were exhausted. When division ended, all cells were capable of growing when sub-cultured, but physiologically were only slightly active. During immersion in oligotrophic waters, cell contents adapted quickly from nutrient-rich to nutrient-limited growth conditions. During adjustment to equilibrium with natural conditions, the three species became impoverished mainly in phosphorus (67–88% of initial values), ATP (54–92%), and chlorophyll a (59–89%); losses of nitrogen were lower (24–53%). To obtain algal material similar to that in the wild, the data suggest that the cells have to be kept in situ only until they divide five times, which usually requires no more than a week. Counting the cell density each day is probably the most convenient method of control, and each culture should be counted, because the difference of growth between aliquot dialysis cultures can be important (up to 28%). Data obtained for “wild-like” cells of Platymonas suecica, Phaeodactylum tricornutum, Thalassiosira pseudonana, and a wild contaminant population of Cylindrotheca closterium Rabenhorst with biochemical analysis used to run the Droop's model and with enrichment bioassays demonstrate that, during the period studied, phosphorus was the limiting factor for the algal growth. Bioassays also demonstrate that there were some specific variations; some algae were permanently limited by phosphorus, while for a few others nitrogen and phosphorus could be equally limiting.