In-situ cell density monitoring and apoptosis detection in adherent Vero cell bioreactor cultures

Emma Petiot,Hervé Pinton,Geoffrey Esteban,Annie Marc,Cécile Gény,Amal El-Wajgali

doi:10.1186/1753-6561-5-s8-p8

Abstract

Background In cell-based processes, and particularly in viral vaccine production, cell growth and death are strategic informations to obtain for process monitoring (ie. scale-up, determination of MOI, TOI, and harvest time). Dielectric spectroscopy is a tool which was increasingly implemented on cell-culture bioreactors as it presents great potentials, compared to other methods, for the in-line monitoring of these two crucial parameters. Considering viral vaccine production, Vero cells are one of the most employed cell platform. But, due to its adherent characteristics, few in-line techniques were developed for cell density monitoring, on the contrary to the ones available for suspension cells. In addition, it should be underline that no in-line technique exists for quantification or detection of the mammalian cell death, despite the importance of this parameter for cell culture processes.

Highlights

In cell-based processes, and in viral vaccine production, cell growth and death are strategic informations to obtain for process monitoring
Dielectric properties of adherent Vero cells A valid correlation between biovolume and cell concentration was observed for batch cultures performed in both media, confirming that the Schwan model [3], originally developed for spherical cells, was implementable to cells attached on microcarrier surface
In the modified cell culture medium, the slope was lower suggesting a reduction of specific permittivity, and so potential changes in cell dielectric properties (CM and si) (Figure 1)

Summary

Introduction

In cell-based processes, and in viral vaccine production, cell growth and death are strategic informations to obtain for process monitoring (ie. scale-up, determination of MOI, TOI, and harvest time). In cell-based processes, and in viral vaccine production, cell growth and death are strategic informations to obtain for process monitoring Scale-up, determination of MOI, TOI, and harvest time). Dielectric spectroscopy is a tool which was increasingly implemented on cell-culture bioreactors as it presents great potentials, compared to other methods, for the in-line monitoring of these two crucial parameters. Considering viral vaccine production, Vero cells are one of the most employed cell platform. Due to its adherent characteristics, few in-line techniques were developed for cell density monitoring, on the contrary to the ones available for suspension cells. It should be underline that no in-line technique exists for quantification or detection of the mammalian cell death, despite the importance of this parameter for cell culture processes

Methods

Results

Conclusion