Abstract

A simple and rapid procedure for the preparation of fluorescent antibodies with fluorescamine is described. The major advantage of the method is that labeled antibody requires no further purification following conjugation. The utility of the method is illustrated by fluorescent staining of virus-specific cytoplasmic and nuclear antigens, those of vesicular stomatitis virus and adenovirus, respectively. The intensity of fluorescence permits short photographic exposure times, and the stained preparations have retained their fluorescence for more than 9 months.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.