In situ and in vitro evaluation of the bioavailability of rumen-protected methionine with coating prototypes

Abstract

Rumen protected amino acids are supplements that can enhance ruminal performance, yet the coating designed to protect the amino acids might also lead to different effects. Methionine is an essential methyl donor to synthesize protein, and little data exists on the effects of coating materials on its bioavailability. The purpose of this study was to estimate the effect of rumen-protected methionine (RPM) coatings with different ratios of acrylic resin IV (AR), ethyl cellulose (EC), and a mixture of AR and EC (AREC). Fifteen RPMs were prepared according to a single factor design, with 5 proportions each of AR, EC, and AREC to DL-methionine (DL-Met). Twelve hybrid small-tailed Han sheep with rumen fistula were utilized to evaluate in situ escape of RPMs, followed by in vitro abomasum-intestinal release of the RPMs. The results showed a regular variation in both ruminal disappearance and gastrointestinal release of RPMs with different coating prototypes and retention time. The RPMs that were EC and AREC coated presented high bioavailability compared to those with AR. Bioavailability of RPMs was optimal with the 2:20 AREC: DL-Met ratio, when the proportion of AR:EC is 1:1. Additionally, RPMs with a 1:3 ratio of AR:EC confirmed the optimum effect for the RPM of 2:20 AREC: DL-Met. In conclusion, an RPM with a lower AREC ratio coating can achieve better bioavailability and is synergistic to those with EC and AR.