Abstract

Enhanced population growth, rapid industrialization, urbanization and hazardous industrial practices have resulted in the development of environmental pollution in the past few decades. Heavy metals are one of those pollutants that are related to environmental and public health concerns based on their toxicity. Effective bioremediation may be accomplished through “ex situ” and “in situ” processes, based on the type and concentration of pollutants, characteristics of the site but is not limited to cost. The recent developments in artificial neural network and microbial gene editing help to improve “in situ” bioremediation of heavy metals from the polluted sites. Multi-omics approaches are adopted for the effective removal of heavy metals by various indigenous microbes. This overview introspects two major bioremediation techniques, their principles, limitations and advantages, and the new aspects of nanobiotechnology, computational biology and DNA technology to improve the scenario.

Highlights

  • Environmental pollution is increasing rapidly due to urbanization and industrialization

  • The technique is considered cost-effective and it provides a permanent solution that is less expensive compared to other physicochemical methods and has become more prevalent in treating soils contaminated by heavy metals

  • The methods of “in situ” bioremediation are preferred for restoring contaminated soil and water environments (Jørgensen et al, 2007) as they involve the mechanisms of removing target pollutants from the natural environment with the help of the metabolic potential of the microbial systems without the process of excavation of contaminated samples (Fruchter & Demian, 2002)

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Summary

Introduction

Environmental pollution is increasing rapidly due to urbanization and industrialization. Pollution nature, depth of pollution, location and cost are some of the criteria which are taken into consideration while choosing any bioremediation technique (Frutos et al, 2012; Smith et al, 2015). Apart from these criteria, temperature, pH, nutrient and oxygen concentration determines the success of bioremediation. Heavy metal contaminated soil or aquatic system can be remediated with the help of “ex situ” and “in situ” techniques. “Ex situ” techniques are complex and have a higher cost compared to “in situ” techniques This mini-review would focus on various mechanisms of “in situ” and “ex situ” bioremediations of heavy metals by microbial systems. It will focus on the latest systems on machine learning, deep learning and artificial neural networks (ANN) for understanding the mechanisms of bioremediations

Principle of bioremediation
Heavy metals toxicity
Effect of heavy metal on environment
Heavy metal toxicity in human
Microbial bioremediation of heavy metals
Factors affecting bioremediation of heavy metals by microorganisms
Biopile
Windrow
Land-farming
Bioreactor
Intrinsic methodology of bioremediation
Bioattenuation
Bioaugmentation
Biostimulation
Bioslurping
Bioventing
Phytoremediation
Mycoremediation
Cyanoremediation
Nanoparticle associated bioremediation
Gene editing for “ex situ” bioremediation
Biodegradation network for ex situ bioremediation
Computational tool for assessing “ex situ” bioremediation
Multi-omics approach
Findings
Conclusions and future prospects
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