Abstract
Photosynthesis is essential for plant productivity and critical for plant growth. More than 90% of plants have a C3 metabolic pathway primarily for carbon assimilation. Improving crop yields for food and fuel is a major challenge for plant biology. To enhance the production of wheat there is need to adopt the strategies that can create the change in plants at the molecular level. During the study we have employed computational bioinformatics and interactomics analysis of C3 metabolic pathway proteins in wheat. The three-dimensional protein modeling provided insight into molecular mechanism and enhanced understanding of physiological processes and biological systems. Therefore in our study, initially we constructed models for nine proteins involving C3 metabolic pathway, as these are not determined through wet lab experiment (NMR, X-ray Crystallography) and not available in RCSB Protein Data Bank and UniProt KB. On the basis of docking interaction analysis, we proposed the schematic diagram of C3 metabolic pathway. Accordingly, there also exist vice versa interactions between 3PGK and Rbcl. Future site and directed mutagenesis experiments in C3 plants could be designed on the basis of our findings to confirm the predicted protein interactions.
Highlights
Photosynthesis is arguably the most important energy conversion process on earth because the chemical energy it yields is the base of food chains that sustain the overwhelming majority of other life forms
On the basis of 1st product of photosynthesis or modi cations in Calvin-Benson Cycle plants are grouped into three categories: C3 plants, C4 plants, and crassulacean acid metabolism (CAM) plants
For this study, selected proteins involved in C3 metabolic pathway were retrieved from NCBI, and their protein sequences in FASTA were used for a query sequence similarity search
Summary
Photosynthesis is arguably the most important energy conversion process on earth because the chemical energy it yields is the base of food chains that sustain the overwhelming majority of other life forms. Plants utilize atmospheric CO2 to liberate oxygen and synthesize carbohydrates during photosynthesis. It is an event where radiant energy of sunlight is utilized to convert carbon dioxide into photosynthetic byproducts. E study utilized computational bioinformatics and interactomics analysis of C3 metabolic pathway proteins in wheat. Proteins interact with each other as well as with other macromolecules to accomplish their functions within cell; protein-protein interactions are complex and play a crucial role in most biological processes to determine the actual functioning of protein Wet lab approaches such as tandem affinity puri cation mass spectrometry [16], yeast two hybrid [17, 18], and some others are found to enable the mapping of complex protein interactions. Each protein have has an important role, either in CO2 xation to regenerate ribulose 1, 5-bisphosphate or to synthesize starch and sucrose. is study selected only nine of proteins involved in the C3 Pathway. e nine selected protein structures have not yet been determined through wet lab experiment (NMR, X-ray Crystallography) nor available in RCSB Protein Data Bank and UniProt KB
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