Abstract
The last two decades have witnessed a tremendous growth in probiotics and in the numbers of publications on their potential health benefits. Owing to their distinguishing beneficial effects and long history of safe use, species belonging to the Lactobacillus genus are among the most widely used probiotic species in human food and dietary supplements and are finding increased use in animal feed. Here, we isolated, identified, and evaluated the safety of two novel Limosilactobacillus reuteri (L. reuteri) isolates, ATCC PTA-126787 & ATCC PTA-126788. More specifically, we sequenced the genomes of these two L. reuteri strains using the PacBio sequencing platform. Using a combination of biochemical and genetic methods, we identified the two strains as belonging to L. reuteri species. Detailed in silico analyses showed that the two strains do not encode for any known genetic sequences of concern for human or animal health. In vitro assays confirmed that the strains are susceptible to clinically relevant antibiotics and do not produce potentially harmful by-products such as biogenic amines. In vitro bile and acid tolerance studies demonstrated that the two strains have similar survival profiles as the commercial L. reuteri probiotic strain DSM 17938. Most importantly, daily administration of the two probiotic strains to broiler chickens in drinking water for 26 days did not induce any adverse effect, clinical disease, or histopathological lesions, supporting the safety of the strains in an in vivo avian model. All together, these data provide in silico, in vitro and in vivo evidence of the safety of the two novel candidates for potential probiotic applications in humans as well as animals.
Highlights
The term “probiotic” was derived from “pro” (Latin, means “for”) and “bios” (Greek, means “life”) and thereby means “for life”
We isolated two novel Lactobacillus strains from chicken cecum, identified them as L. reuteri and established their safety using various genomic, in vitro, and in vivo studies, supporting their application as potential probiotics for human and animal health
L. reuteri is a subtype of L. fermentum and the two species are indistinguishable at the biochemical level [63]. 16S rRNA identification confirmed that our strains have closest homology to L. reuteri strains
Summary
The term “probiotic” was derived from “pro” (Latin, means “for”) and “bios” (Greek, means “life”) and thereby means “for life”. Probiotics are defined as “live microorganisms that, when administered in adequate amounts, confer a benefit on the host” [1]. There has been an unprecedented growth in the application of probiotics to support health and well-. Safety evaluation of novel L. reuteri strains for probiotic applications did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section
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