Abstract
This study aimed to evaluate the in silico, in vitro, and ex-vivo toxicity of vitexin, the flavonoid 5,7,4'- trihydroxyflavone-8-C-β-glucopyranoside from Waltheria viscosissima. The chemical structure and predicted bioactive properties were also in silico analyzed. The in vitro and ex-vivo assays were performed according to the Ethics Code of the World Medical Association and were approved by the Ethics Committee of University Center of Patos (protocol number: 3.621.284). In silico analysis suggested that the molecule presents good oral bioavailability and good absorption; penetrating biological membranes. The toxicity tests revealed the potential effectiveness of the molecule in cellular protection against free radicals, in addition to possible antimutagenic, anticarcinogenic, antioxidant, antineoplastic, anti-inflammatory, anti-hemorrhagic and apoptosis agonist activity. Hemolytic and genotoxic assessment detected low hemolysis rates in human red blood cells and no cellular toxicity against oral mucosa cells. The data suggest that vitexin is a safe molecule for possible therapeutic application and its toxicity profile indicates viability for future studies.
Highlights
This study aimed to investigate the toxicity of vitexin or (5,7,4'-trihydroxyflavone-8-C-βglucopyranoside), as isolated from W. viscosissima using in silico, in vitro, and ex-vivo testing
Vitexin has a molar mass of 432.38 g/mol and a total of 31 atoms
The results revealed that vitexin presents: nON: 10, nOHNH: 7, and nViolations: 1
Summary
The development of medicines originating from naturally-derived products involves study of a wide variety of plant species, identifying their constituents (phytochemistry), and analyzing the bioactive efficacy of the isolated substances through their interactions with target molecules, to elucidate possible pharmacological mechanisms[1].Financial support: APS, CAPES for financial support and the PhD scholarship; DAF, CAPES for financial support and the PhD scholarship; LVC, CAPES for financial support and the PhD scholarship. Conflicts of interest: The authors declare no conflict of interest. The study was carried out at Universidade Federal da Paraíba (UFPB), João Pessoa, PB, Brasil. Received on October 09, 2020. Accepted on December 04, 2020.
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