Abstract
Cruciferin is the major storage protein in Brassicaceae family oilseeds. The predominant cruciferin isoforms in Arabidopsis thaliana were investigated using homology modeling (HM) for their molecular structures and functional properties. The structure of Brassica napus procruciferin was used as the template for HM to determine the molecular structures and hypervariable regions. Hydrophobicity and electrostatic surface potential distribution on the intradisulfide-containing face (IA) and the interdisulfide-containing face (IE) indicated favorable interfacial and solubility properties. More heat-induced structural changes were predicted for the CruC homotrimer than for the CruA or CruB homotrimers. Structural features that facilitate flavor binding and limit proteolytic digestion were more readily observed in CruA and CruB than in CruC. On the basis of these comparative models, structural differences among cruciferin isoforms and their relevance to potential technofunctionalities were identified. This approach of functional property prediction will link protein structure to utilities and will be valuable in designing proteins for targeted applications.
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