Abstract
The aim of this research was to analyze and compare CRIPSR loci and cas-proteins of Yersinia pseudotuberculosis strains isolated in different territories from patients with various clinical manifestations of pseudotuberculosis.Materials and Methods. Complete genomes of Y. pseudotuberculosis IP329353 (NC_006155) and IP31758 (NC_009708) were obtained from NCBI Nucleotide Database. Strains were isolated from patients with gastroenteritis and systemic infection respectively. Search, identification, and analysis of CRISPR systems were carried out by onlinetools CRISPROne, CRISPRDetect, and CRISPRTarget.Results. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953: array YP1 located near cas-genes, arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers.Conclusion. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific molecular marks of analyzed strains during the study of intra-species variability and evolution of Y. pseudotuberculosis.
Highlights
The aim of this research was to analyze and compare CRIPSR loci and cas-proteins of Yersinia pseudotuberculosis strains isolated in different territories from patients with various clinical manifestations of pseudotuberculosis
CRISPR-локусы представлены набором коротких палиндромных повторов (21-47 пн), разделенных штамм-специфичными спейсерными последовательностями
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Summary
The aim of this research was to analyze and compare CRIPSR loci and cas-proteins of Yersinia pseudotuberculosis strains isolated in different territories from patients with various clinical manifestations of pseudotuberculosis. 1. Структура и принцип функционирования CRISPR-Cas системы первого класса. В своих предыдущих публикация Koskela et al, (2015) и Seecharran et al (2017) охарактеризовали CRISPR-локусы Y. pseudotuberculosis и предположили возможные механизмы приобретения CRISPR/Cas системы и её роль в эволюции бактерии [13, 18]. Изучены полногеномные последовательности штаммов Y. pseudotuberculosis IP329353 и IP31758 из базы данных NCBI Nucleotide (NC_006155 и NC_009708 соответственно). Для идентификации CRISPR-локусов и cas-генов использованы онлайн-приложения CRISPROne: http://omics.informatics.
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