In silico analysis of superoxide dismutase family genes in Ipomoea trifida L.

Abstract

Superoxide dismutase (SOD) is described as a key enzyme in the antioxidant system of plants. It plays a vital role in protecting plants against various biotic and abiotic stresses by scavenging reactive oxygen species (ROS) produced by organisms. The SODs genes family has been documented in several plant species, but its characterization in Ipomoea trifida has not been reported. In the present study, the family of SODs genes present in the genome of I. trifida was comprehensively analyzed. Analysis can be performed using bioinformatics tools, physicochemical characterization of proteins, gene structure analysis (exon/intron), phylogenetic relationships, synteny, and expression profile analysis. We identified seven SODs genes, including four Cu/Zn-SOD (CSD), two Fe-SOD (FSD), and one Mn-SOD (MSD), and based on phylogenetic analysis, the SODs genes were classified and divided into three main groups based on their metal cofactor. Synteny analysis revealed eight pairs among SOD genes sweet potato and Arabidopsis. The in silico expression profile showed that ItfSODs genes were differentially expressed. The results of this study provide relevant information for further research of ItfSODs genes in I. trifida aiming to promote the molecular improvement of sweet potato.