In Silico Analysis of Plantaricin EF that Expressed by Plasmid-Associated Bacteriocin Production Gene of Lactobacillus plantarum IBL-2 for Anti-Candida Agent Potential

Abstract

Lactobacillus plantarum species often harbor several plasmids. These plasmids may encoded important traits such as phages or antibiotics resistances, lactose catabolism and production of proteolytic enzyme and also bacteriocins that named plantaricin. Lactobacillus plantarum IBL-2 that isolated from strawberry of Bali plantation have the highest anti-microorganism activity among the L. plantarum isolate collection of BTCC Indonesia. Only few study carried out to examine the antifungal activity of bacteriocin from L. plantarum. This study focus on anti-Candida activity of plasmid associated with bacteriocin production from L. plantarum IBL-2. The isolates were confirmed by the 16S rRNA analysis by PCR and the phylogenetic tree was built based on references sequences and one outgroups from database. The plantaricin gene screening then performed in plasmid that was isolated from L. plantarum IBL-2 by PCR using five pairs or plantaricin gene primer. The anti-Candida potential of plantaricin observed were analyzed by in silico by docking analysis between the plantaricin and receptors of apoptosis proteins. PlnB and PlnEF were observed in the L. plantarum plasmid. Only PlnEF become the focus of study. Analysis of docking study predicted that PlnEF have interactions with apoptosis proteins regulator in eukaryotic cells. PlnEF that encoded by plasmid of L. plantarum may exert anti-Candida potential through interactions with apoptosis proteins regulator.