In silico Analysis and Homology Modelling of Ribulose-1, 5-Bisphosphate Carboxylase/ Oxygenase in Green Seaweed, Ulva fasciata

Abstract

Here, the RuBisCO protein of the green seaweed, Ulva fasciata was selected to characterize the physicochemical properties and find out the three-dimensional structure that is not available in any structural database. Physicochemical characterization was performed by Expasy’s Protparam server. The computed theoretical isoelectric point (pI) is 5.82 which indicates the acidic property and the aliphatic index of 78.48 shows heat stability. The GRAVY value is -0.263 reflects the protein is hydrophilic and water-soluble. SOPMA was used to predict the secondary structure and 40.50% of residues were form the alpha helix, 20.04% formed extended structure, 11.18% forms beta turn and 28.27% forms random coil structure. The modeling of the 3D structure was performed by the Swiss model and the model was validated by PROCHECK and QMEAN.