In search of lost ergots: phylogenetic re-evaluation of Claviceps species in Japan and their biogeographic patterns revealed-SUPP files

Abstract

Supplementary files. <br />Fig. S1. Cultures of Claviceps spp. Three-point inoculation on petri dishes (52 mm diam) with 5 mL T2 agar (sucrose 100 g, l-asparagine 10 g, yeast extract 0.1 g, KH2PO4 0.25 g, MgSO4·7H2O 0.25 g, FeSO4·7H2O 0.02 g, ZnSO4·7H20 0.015 g, KCI 0.12 g, Ca(NO3)2·4H2O 1.0 g, agar 20 g; pH 5.2, 1000 mL). The plates were incubated in dark at 20 °C for 20 days. The morphology of ergot colonies is an unstable feature as it often changes with age. The colonies of the same isolates were shown with a light blue background. T = ex-type, NT = ex-neotype, ET = ex-epitype. <br />Fig. S2. Maximum likelihood phylogenetic tree based on each gene (LSU, TEF-1α, Mcm7, TUB2 and RPB2). Bootstrap (BS) support values from ML and MP analyses are shown at branch (MLBS/MPBS). MLBS < 50 are not shown. Culture collection number or specimen numbers, country code and host genus name are presented after the species name. Ergots analysed in this study are in bold. Ergots collected in Japan are marked by the yellow boxes. The scale represents the number of nucleotide substitutions per site. T = ex-type, NT = ex-neotype, ET = ex-epitype. Abbreviated country code; ARG = Argentina, AU = Australia, BELG = Belgium, BOTSW = Botswana, BRA = Brazil, CA = Canada, CZ = Czech Republic, FRA = France, GER = Germany, IND = India, JA = Japan, KAZ = Republic of Kazakhstan, LIT = Lithuania, MEX = Mexico, SA = South Africa, SWI = Switzerland, THAI = Thailand, UK = United Kingdom, USA = United States of America, ZW = Zimbabwe. <br />Table S1. Nucleotide sequences of primers used in this study.