Abstract
ObjectiveThe effects of vaccinating 18-day-old chicken embryos with the combination of recombinant Eimeria profilin plus Clostridium perfringens (C. perfringens) NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using an Eimeria maxima (E. maxima)/C. perfringens co-infection NE disease model that we previously developed.MethodsEighteen-day-old broiler embryos were injected with 100 μL of phosphate-buffered saline, profilin, profilin plus necrotic enteritis B-like (NetB), profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101). After post-hatch birds were challenged with our NE experimental disease model, body weights, intestinal lesions, serum antibody levels to NetB, and proinflammatory cytokine and chemokine mRNA levels in intestinal intraepithelial lymphocytes were measured.ResultsChickens in ovo vaccinated with recombinant profilin plus NetB proteins/IMS106 and recombinant profilin plus NetB proteins/IMS101 showed significantly increased body weight gains and reduced gut damages compared with the profilin-only group, respectively. Greater antibody response to NetB toxin were observed in the profilin plus NetB/IMS 106, and profilin plus NetB/IMS 101 groups compared with the other three vaccine/adjuvant groups. Finally, diminished levels of transcripts encoding for proinflammatory cytokines such as lipopolysaccharide-induced tumor necrosis factor-α factor, tumor necrosis factor superfamily 15, and interleukin-8 were observed in the intestinal lymphocytes of chickens in ovo injected with profilin plus NetB toxin in combination with IMS 106, and profilin plus NetB toxin in combination with IMS 101 compared with profilin protein alone bird.ConclusionThese results suggest that the Montanide IMS adjuvants potentiate host immunity to experimentally-induced avian NE when administered in ovo in conjunction with the profilin and NetB proteins, and may reduce disease pathology by attenuating the expression of proinflammatory cytokines and chemokines implicated in disease pathogenesis.
Highlights
Necrotic enteritis (NE) is one of the most important enteric infectious diseases affecting global poultry production with an estimated annual economic loss of more than $2 billion, largely attri butable to increased costs associated with medical treatments and impaired growth performance [1,2]
Effects of vaccination with necrotic enteritis B-like (NetB) proteins plus IMS adjuvants on body weight gains and gut lesions Animals in ovo immunized with profilin+NetB/IMS 106 or
Chickens in ovo immunized with profilin+NetB/IMS 106 or profilin+NetB/IMS 101 showed significantly reduced lesion scores at 2 days post-infection with C. perfringens compare with birds given the phosphatebuffered saline (PBS) control or profilin alone group (Figure 2)
Summary
Necrotic enteritis (NE) is one of the most important enteric infectious diseases affecting global poultry production with an estimated annual economic loss of more than $2 billion, largely attri butable to increased costs associated with medical treatments and impaired growth performance [1,2]. NE is caused by infection with toxinproducing, virulent strains of Clostridium perfringens (C. perfringens) in association www.ajas.info
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
