Abstract
The discovery of green fluorescent protein (GFP) and its development into a genetic tag for monitoring cell fate was transformative for bioscience, and in 2008 the Nobel Prize in Chemistry was awarded to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discoveries. Roger Tsien’s development of GFP into an expansive color palette of fluorescent protein tags allowed for the revolutionary ability to monitor different biological processes simultaneously and paved the way for chemical biologists to see into cells like never before. ACS Chemical Biology collected memories from his colleagues, trainees, friends, and family that portray a creative, incisive, and endlessly curious scientist who is deeply missed. This special issue on biological sensing and imaging would not have been possible without his discoveries and is dedicated to his memory.
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