In:Ge/Cu@calcein polyhedral nanostructure conducted photoelectrochemical biosensor for detection of IFN-gamma

Abstract

A novel photoelectrochemical (PEC) biosensor based on the In:Ge/Cu@calcein polyhedral nanostructure was fabricated for ultrasensitive determination of interferon (IFN)-gamma. The calcein dye could trigger Cu nano-octahedral to transform Cu nano-polyhedral structure and attach orderly to the its surfaces by electrochemical co-deposition, and facilitate photocurrent response dramatically enhancing by five-folds. The In:Ge/Cu@calcein demonstrates excellent PEC performance resulting from the synergistic sensitization of calcein and In:Ge/Cu nanostructure, as well as supplying rich carboxy groups to couple with other biomolecules for simply fabricating biosensor, which has a wide dynamic range (from 50 fg mL −1 to 1 ng mL −1 concentrations of IFN-gamma), and a lower detection limit of 24 fg mL −1 .