Abstract
Abstract Using a femtosecond fluorescence up-conversion microscope, viscosity values in yeast cells (budding, wine, and fission) were estimated based on ultrafast fluorescence dynamics of a fluorescent probe dye, Malachite Green. The decay time of the S1 state of Malachite Green in cytoplasm was determined to be 2.2–2.4 ps at room temperature, which corresponded to the viscosity value of 5–6 cP. Nondestructive estimation of viscosity value in cells (in-cell viscosity) was carried out.
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