In 30 Days from Plasmid to Product: Expression of a Secreted Fusion Protein Using Hek293-EBNA Cells

Abstract

The increasing demand of milUgram amounts of recombinant proteins for high throughput screening forces the development of fast and efficient expression systems. Mammalian expression systems have the advantage of yielding proteins with posttranslational modifications, which is an important feature when using proteins in animal models. Besides the classical cell lines hke CHO or COS the human embryonic kidney cell line HEK293-EBNA is more and more commonly used. This expression system offers the possibiUty to produce recombinant proteins with human glycosylation pattern. This generates less false positives in the screening and therefore saves time and money.